Complement Factor H (Blood)

Testing Laboratory

Auckland Te Toka Tumai | Auckland Hospital

Department

Immunology

Delphic Registration Code (Alpha Code)

CFH

Test Code (Numeric Code)

7043

Laboratory Handling

Phlebotomy

Serum: mark request form as urgent

Transport

Samples need to be received in the laboratory within 1 hour of collection or spun and frozen within that time.

Separating

Separate within an hour of collection and store at -20°C.

Laboratory

LabPlus Immunochemistry

Sample Stability

Separated serum samples are stored at ≤20°C

Synonyms

CFH, Factor H

Turnaround Time

5 weeks

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain (Red Top)

Preferred Sample Volume (ml)

3.5

Minimum Sample Volume (ml)

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain (Red Top)

Preferred Sample Volume (ml)

1

Minimum Sample Volume (ml)

1

Aliquot Instructions

Sample Type

• Serum

Minimum Volume (ml)

3.5

Transport to Lab

Frozen

Additional Referral/Aliquot Information

Allow the sample to clot. Spin at 3000 rpm for 5 minutes, Separate sample within 1 hour of collection.
Send frozen.

Test Method

Radial Immunodiffusion using a Binding Site kit.

Limitations / Interference

Microbial contaminates, haemolysed and very lipaemic samples

Resources

• Immunochemical quantitation of antigens by single radial immunodiffusion
• QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES
• Three-dimensional structure of a complement control protein module in solution
• Structure of complement receptor 2 in complex with its C3d ligand
• Complement factor H gene mutation associated with autosomal recessive atypical hemolytic uremic syndrome
• A common haplotype in the complement regulatory gene factor H (HF1/CFH) predisposes individuals to age-related macular degeneration

Contact Information