Methotrexate (Blood)

Testing Laboratory

Auckland Te Toka Tumai | Auckland Hospital

Department

Chemical Pathology

Delphic Registration Code (Alpha Code)

METT

Test Code (Numeric Code)

0928

Laboratory Handling

Phlebotomy

The request form must state the time interval since the last dose.

Registration

URGENT - Hand deliver to 8100 operator at all times.

Separating

Can also be run on EDTA plasma.
Paediatric gel tubes fine for analysis if centrifuged within 2 hours (in house study).

Sample Stability

Separated sample: 14 days at 2-8°C

Synonyms

Plasma Methotrexate, Serum Methotrexate, Methotrexate level, MTX

Turnaround Time

3 hours

URGENT - Hand deliver to 8100 operator at all times.

Specimen Type

  • Plasma
  • Serum

Specimen Requirements

Tube/Container Type:

Preferred
  • Lithium Heparin - Non-gel (Green Cap)
Acceptable
  • Lithium Heparin PST- Gel (Light Green Cap)
  • Plain (Red Top)
Preferred Sample Volume (ml)

5

Minimum Sample Volume (ml)

4

Paediatric Specimen Requirements

Tube/Container Type:

Preferred
  • Lithium Heparin - Non-gel (Green Cap)
Acceptable
  • Lithium Heparin PST- Gel (Light Green Cap)
  • Plain (Red Top)
Preferred Sample Volume (ml)

0.5

Minimum Sample Volume (ml)

0.5

Additional Specimen Information

0.5 mL Paediatric Microtainer Heparin Blood (Preferred)
0.5 mL Paediatric Microtainer Plain Blood
0.5 mL Paediatric Microtainer PST Blood

Last updated: 26/08/2026