Infinium Methylation EPIC v2.0 – Methylation Profiling (Tissue)

Testing Laboratory

Department

Diagnostic Genetics

Delphic Registration Code (Alpha Code)

MEPR

Test Code (Numeric Code)

4387

Laboratory Handling

Sample Stability

FFPE slides are kept at room temperature.

Associated Tests

• MGMT Promoter Methylation Testing (FFPE Tissue)

Synonyms

Infinium EPIC,
Methylation EPIC,
Solid Tissue(malignant) NGS,
CNS

Turnaround Time

6 weeks

Specimen Type

• Tissue

Specimen Requirements

Tube/Container Type:

Preferred

• Slide(s)

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Slide(s)

Additional Specimen Information

1. An H&E slide clearly marked by a histopathologist including % tumour content . A minimum of 75% tumour content within the targeted area is required.

2. A minimum of 10 unstained FFPE slides (10 micron sections)

3. A minimum of two patient identifiers must match between the request form and the slides provided, including the block number .

Test Method

The Illumina Infinium Methylation EPIC v2.0 assay is a microarray-based method of methylation profiling which covers thousands of CpG loci per sample (~930K). It combines bisulfite conversion of genomic DNA and PCR free whole genome amplification (WGA) with array-based capture and scoring of the CpG loci. Signal intensity is measured with the Illumina iScan in order to generate beta values of the relative degree of methylation at every CpG interrogated.
CNS tumour classifications can be obtained by uploading this raw DNA methylation data to the Heidelberg EPIGNOSTIX classifier, an online platform developed and prospectively tested at Deutsches Krebsforschungszentrum (DKFZ; German Cancer Research Center, Heidelberg, Germany) in a large series of paediatric and adult CNS tumours.
Some cases may be also uploaded to alternative classification algorithms such as the NCI/Bethesda classifier.
Method effective from October, 2024

Contact Information