sFlt-1/PlGF ratio (Blood)

Referring Laboratory

Automation, ALSS, LabPlus, Auckland City Hospital

Testing Laboratory

Department

Chemical Pathology

Delphic Registration Code (Alpha Code)

SFLT

Test Code (Numeric Code)

0119

Synonyms

Placental growth factor ratio, Soluble fms-like tyrosine kinase-1, sFlt, PIGF

This test may be vetted by the laboratory. Please include relevant clinical information on the request form to ensure appropriate tests are approved. See the 'Clinical Information' section on the testing laboratory's test guide for more information on the indications for testing.

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• Plain (Red Top)

Preferred Sample Volume (ml)

4

Requests must be made by SMOs/consultants only
See below for appropriate test indications (i.e. approval criteria). Test requests can be rejected if there are no clear clinical details/indications written on the blood request form or lacking the requesting SMO's details.

Pregnant women in >20 weeks gestation AND one of:
- Systolic blood pressure >= 130 mmHg and/or diastolic blood pressure >= 80 mmHg
- Renal insufficiency, proteinuria, thrombocytopenia, liver dysfunction without hypertension
- Symptoms suggestive of preeclampsia e.g. headache, abdominal pain, epigastric pain or visual field disturbance
- When another alternate diagnosis is being considered e.g. SLE, underlying renal or liver disease
- Suspicion of fetal growth restriction
- One test (sFlt-1/PlGF ratio) per week per patient only

Sendaway to Middlemore Hospital
- This test is routinely available within 24hrs.
- Samples are transported by courier to Middlemore hospital at 9am and 2pm and should usually be available in Eclair within 2-4hr after arrival.
- Turnaround time once arrived at MMH laboratory: within 3 hours from time of receipt.

Urgent requests at other times can be sent by taxi but requires prior approval by a Chemical Pathologist.

Sample stability
- After centrifugation, the separated serum is stable for 48h at 2-8°C
- If sample will arrive at MMH lab within 48h from collection: transport at 2-8°C
- If sample will arrive at MMH lab after 48h or more from collection: transport frozen, -20°C

Sendaway
- Serum (Heparin plasma is not acceptable)
- Centrifuge and aliquot
- Two points of ID on aliquot tube

Monday to Friday: Registered before 7:30 am
- Send to Middlemore Hospital on the 9 am routine courier

Monday to Friday: Registered between 7:30 am and 1:30 pm
- Send to Middlemore Hospital on the 2 pm routine courier

Monday to Friday: Urgent requests after 1:30 pm
- Send to Middlemore Hospital by either Sub60 or taxi

Out-of-hours: Urgent requests
- Send to Middlemore Hospital by taxi

Any queries please contact Section Leader/Technical Specialist of Automation Chemistry

Contact Information