Platelet Function Assay (Blood)

Testing Laboratory

Counties Manukau | Middlemore Hospital

Department

Haematology

Delphic Registration Code (Alpha Code)

PFA

Test Code (Numeric Code)

1820

Laboratory Handling

Phlebotomy

Tube must be filled up to the "Fill Line"

Transport

PFA samples must not be sent via Lamson system (Hand-deliver only)

Registration

Register for FBC (1000) as well

Separating

Do not spin

Sample Stability

Sample must be processed within 4 hours of collection and kept at room temperature at all times

Associated Tests

• Full Blood Count (Blood)
• Coagulation Screen Basic (Blood)

Synonyms

Bleeding Time

Turnaround Time

1 hour

Available 24 hours

Specimen Type

• Plasma
• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• Please refer to Additional Information below
• Citrate (Light Blue Cap)
• EDTA (Lavender Cap)

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Please refer to Additional Information below

Additional Specimen Information

2 x 2.7 mL Citrate tubes (Light blue top). Tubes must be filled up to the "Fill Line". Two tubes is sufficient if a Basic Coagulation Screen is required as well.

3 x 2.7 mL Citrate tubes (Light Blue Top) is required if a von Willebrand screen is required as well.

Limitations / Interference

Sample must be processed within 4 hours of collection and kept at room temperature at all times. If the sample has been spun for clotting screen, then it cannot be used for PFA.

Uncertainty of Measurement

Collagen/EPI: 22%, Collagen/ADP:15%

Additional Costing Information

For pricing inquiries, please contact us at 092760167

The PFA-200 system provides a quantitative measure of platelet function in anticoagulated whole blood.

It simulates the process of platelet adhesion and aggregation following vascular injury. The system uses cartridges with a membrane coated with collagen and either epinephrine or ADP. Blood is drawn through a capillary and aperture cut into the membrane at high shear stress, mimicking conditions in the normal circulation. Platelets adhere and aggregate at the aperture, building a stable platelet plug and finally arresting the blood flow. The analyser measures time to occlusion. This is reported as the closure time in seconds.

Contact Information