Routine (Formalin Fixed)

Testing Laboratory

Department

Histology

Delphic Registration Code (Alpha Code)

MSRG

Test Code (Numeric Code)

1234

Laboratory Handling

Transport

Formalin fixed can be transported at ambient temperature: 22-25 °C. Place specimens into 10% Neutral Buffered Formalin (NBF) immediately after removal to prevent autolysis. Select a wide-mouth, leak proof specimen container appropriate for the specimen size. The minimum fixative-to-tissue ratio is 10:1 (fixative:tissue) required to ensure proper uniform fixation and prevent autolysis. Ensure all lids are tightly secured and checked leaks before bagging. Place the primary container into a sealed biohazard bag.

Sample Stability

Routine histology (formalin fixed) specimens are stable at ambient/room temperature.

Synonyms

Anatomical Pathology
Biopsies
Paraffin Block
H&E Tissue

Turnaround Time

5 days

Routine histology: 80% within 5 working days, 90% within 10 working days, 98% within 15 working days. Turnaround times will vary based on the complexity of the sample and resourcing of the laboratory. Please indicate if the specimen is urgent or if there is a clinic date when results are needed.

Specimen Type

• Biopsy
• Tissue

Specimen Requirements

Tube/Container Type:

Preferred

• Specimen Container

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Specimen Container

Pre-testing Requirements

Labelling requirements: Both container and the request form must be labelled with at least two patient identifiers (e.g., Full name, date of birth or NHI number). Site of specimen is also required.

Specimen container must be accompanied by a requisition form detailing relevant clinical details, site, and any reference to relevant previous Histology Pathology would be appreciated.

Additional Specimen Information

Tissue should be immersed in 10 times its volume of 10% Buffered Formalin.

Formalin is a hazardous chemical - appropriate measures should be taken when dealing with it. Tightly secure the container lid to ensure no leaks.

Specimen containers and formalin can be ordered directly from APS by completing the supply order form in the resources below.

Test Method

Fixation
Macroscopic Examination
Tissue Processing
Embedding and Microtomy
Routine Staining
Microscopic interpretation

Limitations / Interference

The most common errors are preanalytical factors before the specimen reaches the lab:
Patient labelling error
Specimen type not indicated
Inappropriate fixative
Sampling error
Insufficient clinical information
Inadequate responsible clinician information

Uncertainty of Measurement

Uncertainty of measurement is not applicable to routine histology as this is a qualitative, interpretative test.

A number of supplies for specimen testing at APS are supplied (e.g. specimen containers, formalin, request pads etc.). An editable copy of the order form can be found in the resources section below. Clearly indicate the doctor or practice name and address for delivery. Orders of APS request form pads should be placed by 5pm Tuesday for delivery the following week. Email completed forms back to AK-aps.info@TeWhatuOra.govt.nz

Supply orders are transported via Awanui Couriers, allow 2-3 days for delivery - please note, no urgent service is provided.

Resources

• APSMGMTCUSTFO001 APS Supply Order Form
• APSMGMTCUSTFO003 APS Supply Order Instructions
• APSMGMTPPIN003 Notification 1L Containers - securing lid
• APSMGMTCUSTIN011 Histology Sample Checklist
• APSMGMTCUSTIN012 Eligibility Requirements for Laboratory Referrals

Contact Information