Diagnostic Use
When interpreting glucose results, individual biological variation should be taken into account. The within-subject biological variation for glucose is 5% (4) and with 95% confidence interval, the variation would be +/- 10% within an individual. For example, a fasting glucose of 6.0 mmol/L means that it can range from 5.4 – 6.6 mmol/L within that individual.
Furthermore, there are other factors affecting the glucose levels including acute illness, stress, medications, diet patterns, physical activity, etc (5).
Stated fasting glucose ranges are provided as a guide to interpretation in clinically stable patients, and should not be used for diagnosis if the patient is acutely unwell, as glucose tolerance can be temporarily significantly impaired. Equivocal results, especially near the diagnostic cut-offs, should be followed-up when patient is clinically stable.
For random (untimed) samples, a glucose result >11 mmol/L in a stable well patient is diagnostic of diabetes if symptomatic. However, the result should be confirmed with a followup glucose and also HbA1c if they are asymptomatic.
An elevated glucose does not necessarily establish the diagnosis if the patient is acutely unwell and especially if on drugs which impair glucose tolerance such as steroids. Status may need to be confirmed once clinical status has stabilised after 3-6 months.
For diagnosis of diabetes mellitus, HbA1c has the advantage of less biological variation (1.2% in healthy subjects) (4) and is less affected by acute changes (6, 7).
Test Method
Principle: Hexokinase
Reagents: Siemens Atellica CH Glucose Hexokinase
Analyser: Siemens Atellica CH