17-Hydroxy Progesterone (Blood)

Testing Laboratory

Department

Chemical Pathology

Delphic Registration Code (Alpha Code)

TOHP

Test Code (Numeric Code)

3951

Laboratory Handling

Transport

Send refrigerated up to 4 days
Send frozen if more than 4 days

Separating

Centrifuge and aliquot - 500uL
Freeze

Laboratory

LabPLUS High Performance Liquid Chromatography

Sample Stability

Room temperature up to 24 hours
Refrigerated up to 4 days
Freeze if more than 4 days

Associated Tests

• 11-Deoxycortisol (Blood)
• Androstenedione (Blood)

Synonyms

17 OHP
17OHP
OHP
Hydroxyprogesterone
17-hydroxyprogesterone

Turnaround Time

1 week

Performed weekly
If an urgent test is required for CAH in babies, National Testing Centre can test a dried blood spot. Analysed daily, or urgently if required. Contact laboratory.

Specimen Type

• Plasma
• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• Plain (Red Top)

Acceptable

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

4

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Plain(Microtainer)

Acceptable

• EDTA (Microtainer)

Preferred Sample Volume (ml)

0.5

Aliquot Instructions

Sample Type

• Serum
• Plasma

Transport to Lab

Chilled (2 - 8 degrees Celsius)
Frozen

Additional Referral/Aliquot Information

Transport to LabPlus:
Send refrigerated up to 4 days
Send frozen if more than 4 days

Test Method

Liquid Chromatography Mass Spectrometry (LC-MS/MS)

Uncertainty of Measurement

< 5nmol/L 26%
>= 5 nmol/L 22%

References:

1. Pang S. Congenital adrenal hyperplasia. Endocrinol & Metabolism Clinics of N America 26 (1997) 853-891.

2. Soldin, O. P., H. Sharma, et al. (2009). "Pediatric reference intervals for aldosterone, 17alpha-hydroxyprogesterone, dehydroepiandrosterone, testosterone and 25-hydroxy vitamin D3 using tandem mass spectrometry." Clin Biochem 42 (9): 823-827.

3. Fanelli, F., A. Gambineri, et al. (2013). "Androgen profiling by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in healthy normal-weight ovulatory and anovulatory late adolescent and young women." J Clin Endocrinol Metab 98 (7): 3058-3067.

4. Rauh, M., M. Groschl, et al. (2006). "Automated, fast and sensitive quantification of 17 alpha-hydroxy-progesterone, androstenedione and testosterone by tandem mass spectrometry with on-line extraction." Steroids 71 (6): 450-458.

5. Kulle, A. E., M. Welzel, et al. (2013). "Implementation of a liquid chromatography tandem mass spectrometry assay for eight adrenal C-21 steroids and pediatric reference data." Horm Res Paediatr 79 (1): 22-31.

6. Kyriakopoulou, L., M. Yazdanpanah, et al. (2013). "A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals." Clin Biochem 46 (7-8): 642-651.

7. Costa-Barbosa, F. A., V. F. Tonetto-Fernandes, et al. (2010). "Superior discriminating value of ACTH-stimulated serum 21-deoxycortisol in identifying heterozygote carriers for 21-hydroxylase deficiency." Clin Endocrinol (Oxf) 73 (6): 700-706.

Contact Information