ADAMTS-13 Activity with Inhibitor Screen (Blood)

Testing Laboratory

Department

Haematology

Delphic Registration Code (Alpha Code)

TS13

Test Code (Numeric Code)

1365

Laboratory Handling

Phlebotomy

Synonyms

ADAMTS-13 Level
ADAMTS-13

Turnaround Time

3 days

Requests for ADAMTS-13 activity must be arranged through a LabPlus Haematologist who will also advise when results will be available. Please note the assay takes 5 hours to complete.

This test may be vetted by the laboratory. Please include relevant clinical information on the request form to ensure appropriate tests are approved. See the 'Clinical Information' section below for more information on the indications for testing.

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• Citrate (Blue Cap)

Preferred Sample Volume (ml)

2.7

No of Samples Required

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Citrate (Microtainer)
• Please refer to Additional Information below

Minimum Sample Volume (ml)

2

Pre-testing Requirements

Requests for this test must be arranged by contacting a LabPLUS Haematologist.
Requests must be accompanied by the CC7719 ADAMTS13 Request Form or it will not be processed.
CC7719 ADAMTS13 Request Form Link
Please provide requestor contact phone details on the request form.

Additional Specimen Information

Paediatric: collect 2 x 1ml Citrate microtainer.
1. EDTA destroys ADAMTS-13 activity, so it is important to collect/fill citrate specimen(s) before EDTA specimens to avoid EDTA contamination.
2. Collection tubes must be adequately filled.

Aliquot Instructions

Sample Type

• Plasma

Transport to Lab

Frozen

Additional Referral/Aliquot Information

Double spin plasma before freezing in 1 ml aliquots.
To ensure the plasma is platelet poor before freezing double spin plasma as follows: transfer the plasma after the initial centrifugation to a suitable plastic tube using a plastic transfer pipette then re-centrifuge. When aliquoting from the second spin tube into the final tube take care to avoid the bottom 0.5 cm where residual platelets are deposited. Identify double spun aliquots by noting this on the label.
Haemolysed specimens may be unsuitable for testing.

Test Method

ELISA method.
NOTE: The ADAMTS-13 activity is reported together with an ADAMTS-13 inhibitor screen for timely distinction between congenital and acquired deficiency.

Uncertainty of Measurement

Please contact relevant Pathologist to discuss sources of test uncertainty such as but not limited to measurement uncertainty and biological variation.

Resources

• ADAMTS13 Request Form

Contact Information