Chromosome analysis – Karyotype (Leukaemic Blood)

Testing Laboratory

Department

Diagnostic Genetics

Delphic Registration Code (Alpha Code)

DGEN

Test Code (Numeric Code)

4218

Laboratory Handling

Registration

Place the sample into the box labelled DGEN on the shelf next to the dumbwaiter.

Sample Stability

All samples must be collected using sterile technique and same day delivery (8-24 degrees C).

Associated Tests

• Chromosome analysis – Karyotype (Bone Marrow)
• Chromosome analysis – Solid tumours (Tissue)

Synonyms

Karyotype, leukaemic blood
Leukaemia blood chromosomes
Bone Marrow Chromosomes,
Bone Marrow/Blood Cancer Cytogenetics,
Cytogenetics
G-banded karyotype

Turnaround Time

18 days

Urgent Cases 5 Days

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• Lithium Heparin - Non-gel (Green Cap)

Preferred Sample Volume (ml)

4

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Lithium Heparin - Non-gel (Green Cap)

Preferred Sample Volume (ml)

4

Additional Specimen Information

Whole blood is required for genetic testing; please do not centrifuge blood tubes.
Do NOT refrigerate or freeze
Ideally send same day or overnight

Additional Referral/Aliquot Information

Analysis and interpretation of this assay require accurate knowledge of the patient’s biological sex. To support the delivery of high-quality results, please ensure the patient’s biological sex is correctly recorded on all referrals for this test.

Test Method

Samples are cultured and processed for G-banded Karyotype analysis and/or FISH testing. This provides whole-genome analysis detecting both numerical and structural abnormalities and permits the identification of clonal evolution and the presence of multiple clones.

The number of cells analysed and counted are based on the NPAAC guidelines.

Limitations: micro- deletions/duplications (below 5-10MB) and cryptic translocations may not be excluded.

Uncertainty of Measurement

Please contact the Diagnostic Genetics Pathologist at dgen@adhb.govt.nz to discuss sources of test uncertainty, which include, but are not limited to, measurement uncertainty (MU) and biological variation.

Contact Information