Fluorescence In Situ Hybridisation (FISH) – Postnatal (Peripheral Blood)

Testing Laboratory

Department

Diagnostic Genetics

Delphic Registration Code (Alpha Code)

DGEN

Test Code (Numeric Code)

4218

Laboratory Handling

Registration

Place the sample into the box labelled DGEN on the shelf next to the dumbwaiter.

Sample Stability

Specimens to be kept at room temperature (>8 degrees Celsius to <24 degrees Celsius) and sent to LabPlus urgently.

Associated Tests

• Chromosome analysis – Karyotype (Peripheral Blood)

Synonyms

FISH,

Turnaround Time

18 days

Urgent: 5 days

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• Lithium Heparin - Non-gel (Green Cap)

Acceptable

• Lithium Heparin PST- Gel (Light Green Cap)

Preferred Sample Volume (ml)

4

Minimum Sample Volume (ml)

2

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Lithium Heparin - Non-gel (Green Cap)

Acceptable

• Heparin (Microtainer)

Preferred Sample Volume (ml)

2

Minimum Sample Volume (ml)

1

Additional Specimen Information

Clinical details are essential so that the degree of urgency can be assessed and the most appropriate probe(s) can be applied.

Aliquot Instructions

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Test Method

FISH is a technique in which fluorescently labelled DNA probes are hybridised to target DNA on a slide. Interphase and/or metaphase cells are routinely analysed.
The number and quality of cells examined are based on the NPAAC guidelines, unless otherwise stated.

Limitations / Interference

FISH findings should never be used in isolation to establish a diagnosis. Any signal detected below laboratory threshold is not reported. FISH studies target specific chromosome sequences; therefore, the probes used in this study will only detect abnormalities at the loci specified.

Uncertainty of Measurement

Please contact relevant Pathologist to discuss sources of test uncertainty such as but not limited to measurement uncertainty and biological variation.

Contact Information