HBV Drug Resistance Genotyping

Testing Laboratory

Department

Virology

Delphic Registration Code (Alpha Code)

HBYM

Test Code (Numeric Code)

8986

Laboratory Handling

Phlebotomy

A dedicated specimen (i.e. no other tests to be performed) is required for this assay. PPT is the preferred specimen type. Plasma separated from EDTA whole blood, including EDTA Micro collect tubes, are acceptable

Separating

PPT tubes must be centrifuged and separated within 6 hours of collection. EDTA tubes must be centrifuged within 6 hours of collection and the plasma transferred to a separate sterile tube. Send a minimum of 1.0 mL of separated plasma.

Laboratory

Separated plasma samples: should be sent to the Molecular Infectious Diseases section during normal working hours, Monday to Friday (08:00–16:00). After hours and on weekends, store specimens at 2–8°C in the designated box/rack in ALSS.

Sample Stability

PPT tubes with separated plasma are stable at 2–8°C for up to 7 days. If storage exceeds 7 days, plasma should be aliquoted and frozen at ≤−20°C. Plasma aliquots separated from EDTA whole blood are stable at 2–8°C for up to 7 days. If storage exceeds 7 days, aliquots should be frozen at ≤−20°C.

Associated Tests

• HBV Viral Load

Synonyms

Hepatitis B Virus (HBV) Drug Resistance
HBV Antiviral Resistance
HBV Genotyping

Turnaround Time

1 week

Tests performed as required.

Specimen Type

• Plasma
• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• PPT Plasma Preparation Tube (Pale Pink Cap)

Acceptable

• PPT Plasma Preparation Tube (Pale Pink Cap)

Preferred Sample Volume (ml)

2

Minimum Sample Volume (ml)

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Microtainer)

Acceptable

• EDTA (Microtainer)

Preferred Sample Volume (ml)

2

Minimum Sample Volume (ml)

1

Pre-testing Requirements

A recent viral load result obtained within the past month, with a minimum viral load of 3 log₁₀ IU/mL, is required.

Additional Specimen Information

Urgent enquiries:
In normal working hours (08:00-16:00 Mon-Fri) contact Clinical Virology via LabLINK (internal extension 22000, external number 09 307 8995).
Outside of normal working hours (evenings and weekends) contact the On Call Clinical Microbiologist via Te Whatu Ora Te Toka Tumai Auckland main switchboard (09 367 0000).
Non-urgent enquiries:
Email: AK-MOL-INF-DIS@tewhatuora.govt.nz

Aliquot Instructions

Sample Type

• Serum
• Plasma

Minimum Volume (ml)

1

Transport to Lab

Chilled (2 - 8 degrees Celsius)
Frozen

Additional Referral/Aliquot Information

Sample requirements:
PPT is the preferred specimen type. Plasma separated from EDTA whole blood, including EDTA Micro collect tubes, are acceptable.
PPT tube must be centrifuged and plasma separated within 6 hours of collection.
EDTA tubes must be centrifuged within 6 hours of collection and the plasma transferred to a separate sterile tube.
Submit a minimum of 1.0 mL of separated plasma.

Sample stability:
PPT tubes with separated plasma are stable at 2–8°C for up to 7 days. If storage exceeds 7 days, plasma should be aliquoted and frozen at ≤−20°C.
Plasma aliquots separated from EDTA whole blood are stable at 2–8°C for up to 7 days. If storage exceeds 7 days, aliquots should be frozen at ≤−20°C.

Transport:
Transport separated plasma chilled at 2–8°C if it will be received within 7 days of collection. If this period will be exceeded, transport the specimen frozen.

Test Method

Polymerase chain reaction (PCR) amplification of polymerase gene, followed by Sanger sequencing to detect resistance-associated mutations.

Urgent enquiries:
In normal working hours (08:00-16:00 Mon-Fri) contact Clinical Virology via LabLINK (internal extension 22000, external number 09 307 8995).
Outside of normal working hours (evenings and weekends) contact the On Call Clinical Microbiologist via Te Whatu Ora Te Toka Tumai Auckland main switchboard (09 367 0000).
Non-urgent enquiries:
Email: AK-MOL-INF-DIS@tewhatuora.govt.nz

Contact Information