Methotrexate

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Testing Laboratory

Auckland Te Toka Tumai | Auckland Hospital

Department

Chemical Pathology

Delphic Registration Code (Alpha Code)

METT

Test Code (Numeric Code)

0928

Laboratory Handling

Phlebotomy

The request form must state the time interval since the last dose.

Registration

URGENT - Hand deliver to 8100 operator at all times.

Separating

Paediatric gel tubes fine for analysis if centrifuged within 2 hours (in house study)

Test Adds Requirement

Can also be run on EDTA plasma. Paediatric gel tubes fine for analysis if centrifuged within 2 hours (in house study)

Sample Stability

-

Synonyms

Plasma Methotrexate
Serum Methotrexate

Turnaround Time

3 hours

URGENT - Hand deliver to 8100 operator at all times.

Specimen Type

  • Plasma
  • Serum

Specimen Requirements

Tube/Container Type:

Preferred
  • Lithium Heparin - Non-gel (Green Cap)
Acceptable
  • Plain (Red Top)

Paediatric Specimen Requirements

Tube/Container Type:

Preferred
  • Heparin (Microtainer)
Acceptable
  • Plain(Microtainer)

Additional Specimen Information

Heparin 5 mL Heparin Blood (Preferred)
Micro-heparin 0.5 mL Paediatric Micro-heparin Blood (Preferred)
Plain 4 mL Plain Blood
Microsample 0.5 mL Paediatric Microsample Blood
PST 4 mL Adult PST Plasma
Micro-PST 0.5 mL Paediatric Micro-PST Plasma

Last updated: 31/03/2026