Myeloperoxidase Antibodies (Blood)

Testing Laboratory

Auckland Te Toka Tumai | Auckland Hospital

Department

Immunology

Delphic Registration Code (Alpha Code)

MPO

Test Code (Numeric Code)

7173

Laboratory Handling

Phlebotomy

Serum

Registration

MPO

Laboratory

LabPlus Autoimmune Serology

Sample Stability

Up to 48 hours: 8 - 24 °C; Up to 14 days: 4 - 8 °C; Up to 6 months: -30 °C; Indefinite at - 80 °C

Associated Tests

• Anti-Neutrophil Cytoplasmic Antibodies (Blood)
• URGENT Vasculitis (Blood)
• Proteinase 3 Antibodies (Blood)

Synonyms

MPO-ANCA
MPO
Anti-MPO
Anti-Myeloperoxidase antibodies
Neutrophil Cytoplasmic Antibodies
Vasculitis antibodies

Turnaround Time

3 days

Routine requests: 2-3 days; Urgent requests: 3.5 hours

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain (Red Top)

Preferred Sample Volume (ml)

3.5

Minimum Sample Volume (ml)

1

No of Samples Required

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain(Microtainer)
• SST(Microtainer)

Minimum Sample Volume (ml)

1

Aliquot Instructions

Sample Type

• Serum

Minimum Volume (ml)

0.5

Transport to Lab

Chilled (2 - 8 degrees Celsius)

Test Method

Multiplex immunoassay (Luminex)
Routine requests are screened by indirect immunofluorescence and specific antibody tests for PR-3-ANCA and MPO-ANCA are performed on positive samples.

Limitations / Interference

Grossly haemolysed and/or high lipaemic serum specimens.

Uncertainty of Measurement

This information is available upon request

References:

1. FIDIS - Vasculitis product insert.
2. Bossuyt, X., Cohen Tervaert, JW., Arimura, Y. et al. Revised 2017 international consensus on testing of ANCAs in granulomatosis with polyangiitis and microscopic polyangiitis. Nat Rev Rheumatol 13, 683–692 (2017). https://doi.org/10.1038/nrrheum.2017.140

Contact Information