PR3-ANCA (Anti-PR3)

Testing Laboratory

Department

Immunology

Delphic Registration Code (Alpha Code)

PR3

Test Code (Numeric Code)

7172

Laboratory Handling

Phlebotomy

Serum

Registration

PR3

Sample Stability

Up to 48 hours: 8 - 24 °C; Up to 14 days: 4 - 8 °C; Up to 6 months: -30 °C; Indefinite at - 80 °C

Associated Tests

• ANCA (Anti-Neutrophil Cytoplasmic Antibody)
• MPO-ANCA (Anti-MPO)

Synonyms

Anti-Proteinase 3 antibodies
cANCA
Neutrophil Cytoplasmic Antibodies
Granulomatosis with Polyangiitis (GPA)
Wegener’s granulomatosis

Turnaround Time

3 days

Routine requests: 2-3 days; Urgent requests: 3.5 hours

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain (Red Top)

Preferred Sample Volume (ml)

3.5

Minimum Sample Volume (ml)

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain(Microtainer)
• SST(Microtainer)

Minimum Sample Volume (ml)

1

Additional Specimen Information

INDICATIONS FOR REQUESTING TEST

1. Chronic destructive disease of the upper airways
2. Pulmonary nodules, non-malignant
3. Subglottic stenosis of the trachea
4. Pulmonary-renal syndrome
5. Glomerulonephritis
6. Suspected vasculitis
7. Mononeuritis multiplex
8. Retro-orbital mass
NOTE: This testing can be requested as routine where there is a low pre-test probability of a small vessel vasculitis (SVV) or as an urgent request where there is a high probability of a small vessel vasculitis and delivery of results will immediately change therapy for the patient.

Aliquot Instructions

Sample Type

• Serum

Minimum Volume (ml)

0.5

Transport to Lab

Chilled (2 - 8 degrees Celsius)

Additional Referral/Aliquot Information

This testing can be requested as routine where there is a low pre-test probability of a small vessel vasculitis (SVV) or as an urgent request where there is a high probability of a small vessel vasculitis and delivery of results will immediately change therapy for the patient.

Test Method

Multiplex immunoassay (Luminex)

Routine requests:
Serum are screened by indirect immunofluorescence (IIF), and specific antibody tests for PR-3 and MPO are performed on positive samples using luminex methodology.

If there is nothing documented on the request form to indicate either "rapid" or "urgent" then treat as a routine request.

Turnaround time: 2-3 days (Negative result), within 1 week for IIF reactive sera IIF C-ANCA IIF patterns are strongly associated with GPA [Granulomatosis with Polyangiitis, previously Wegener's Granulomatosis] (positive in 85 to 100% of patients with active generalised disease, less frequently in patients with limited disease). The target antigen giving a C-ANCA IIF pattern is PR3 (Proteinase 3).

P-ANCA IIF patterns are seen in the following SVV's:- MPA microscopic polyangiitis, idiopathic necrotising and crescentic glomerulonephritis (pauci-immune GN), EGPA (Eosinophilic Granulomatosis with Polyangiitis, previously Churg-Strauss syndrome). However, they can also be present in several non-SVV related diseases. The target antigen giving a P-ANCA pattern in a SVV disease is MPO (Myeloperoxidase).

In patients not under treatment with a known SVV, a negative IIF result (both patterns) excludes an ANCA-mediated vasculitic disease.

Limitations / Interference

Grossly haemolysed and/or high lipaemic serum specimens.

Uncertainty of Measurement

20%

References:

1. FIDIS - Vasculitis product insert.

Contact Information