ANCA (Anti-Neutrophil Cytoplasmic Antibody)

Testing Laboratory

Department

Immunology

Delphic Registration Code (Alpha Code)

NEUT

Test Code (Numeric Code)

7155

Laboratory Handling

Phlebotomy

Serum

Transport

Ambient (8 - 24°C) within 48 hours; Chilled (4 - 8°C) if longer than 48 hours

Registration

NEUT

Sample Stability

Up to 48 hours: 8 - 24°C; Up to 14 days: 4 - 8°C; Up to 6 months: -30°C; Indefinite at - 80°C

Associated Tests

• PR3-ANCA (Anti-PR3)
• MPO-ANCA (Anti-MPO)
• URGENT Vasculitis (ANCA) Screen

Synonyms

ANCA

Turnaround Time

3 days

Between 2 to 3 days

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain(Microtainer)
• SST(Microtainer)
• Plain (Red Top)

Preferred Sample Volume (ml)

3.5

Minimum Sample Volume (ml)

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• SST II (Gold Cap)

Acceptable

• Plain(Microtainer)
• SST(Microtainer)
• Plain (Red Top)

Minimum Sample Volume (ml)

1

Additional Specimen Information

Paediatric Specimen: 2 mL clotted blood or 1 mL serum

Aliquot Instructions

Sample Type

• Serum

Transport to Lab

Ambient (8 - 24 degrees Celsius)
Chilled (2 - 8 degrees Celsius)

Additional Referral/Aliquot Information

Ambient (8 - 24°C) within 48 hours; Chilled (4 - 8°C) if longer than 48 hours

Test Method

Indirect Immunofluorescence Assay (IFA) - add brand
Positive results are automatically tested for anti-PR3 and anti-MPO by multiplex immunoassay (Luminex)

Limitations / Interference

Grossly haemolysed and/or high lipaemic serum specimens.

Uncertainty of Measurement

Not applicable for IFA

Contact Information